PHLDA1 Mouse Monoclonal Antibody [Clone ID: OTI4D9]

CAT#: CF810937

Carrier-free (BSA/glycerol-free) PHLDA1 mouse monoclonal antibody,clone OTI4D9

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHLDA1 (RC220155, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PHLDA1 (1:2000). Positive lysates LY402133 (100ug) and LC402133 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-PHLDA1 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI4D9
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 183-401 of human PHLDA1 (NP_031376) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44.8 kDa
• Gene Name: Homo sapiens pleckstrin homology like domain family A member 1 (PHLDA1), mRNA.
• Database Link:
  NP_031376
  Entrez Gene 29380 RatEntrez Gene 22822 Human
  UniProt ID Q8WV24
• Synonyms: DT1P1B11; PHRIP; TDAG51