PTF1A Mouse Monoclonal Antibody [Clone ID: OTI5E5]

CAT#: CF810945

Carrier-free (BSA/glycerol-free) PTF1A mouse monoclonal antibody,clone OTI5E5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTF1A (RC221496, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTF1A. Positive lysates LY406006 (100ug) and LC406006 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI5E5
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PTF1A (NP_835455) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.8 kDa
• Gene Name: Homo sapiens pancreas associated transcription factor 1a (PTF1A), mRNA.
• Database Link:
  NP_835455
  Entrez Gene 19213 MouseEntrez Gene 117034 RatEntrez Gene 256297 Human
  UniProt ID Q7RTS3
• Background: This gene encodes a protein that is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers. [provided by RefSeq, Jul 2008]
• Synonyms: bHLHa29; PACA; PAGEN2; PTF1-p48

Reference Data

• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS