ZNF454 Mouse Monoclonal Antibody [Clone ID: OTI2G4]

CAT#: CF810948

Carrier-free (BSA/glycerol-free) ZNF454 mouse monoclonal antibody,clone OTI2G4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF454 (RC219718, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF454 (1:2000). Positive lysates LY405474 (100ug) and LC405474 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2G4
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombiant protein of human ZNF454 (NP_872400) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 59.9 kDa
• Gene Name: Homo sapiens zinc finger protein 454 (ZNF454), transcript variant 2, mRNA.
• Database Link:
  NP_872400
  Entrez Gene 285676 Human
  UniProt ID Q8N9F8
• Synonyms: FLJ37444; MGC138481

Reference Data

• Protein Families: Transcription Factors