RNF11 Mouse Monoclonal Antibody [Clone ID: OTI1G7]

CAT#: CF810976

Carrier-free (BSA/glycerol-free) RNF11 mouse monoclonal antibody,clone OTI1G7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF11 (RC208220, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF11. Positive lysates LY402321 (100ug) and LC402321 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1G7
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 2-154 of human RNF11 (NP_055187) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 17.3 kDa
• Gene Name: Homo sapiens ring finger protein 11 (RNF11), mRNA.
• Database Link:
  NP_055187
  Entrez Gene 26994 Human
  UniProt ID Q9Y3C5
• Background: The protein encoded by this gene contains a RING-H2 finger motif, which is known to be important for protein-protein interactions. The expression of this gene has been shown to be induced by mutant RET proteins (MEN2A/MEN2B). The germline mutations in RET gene are known to be responsible for the development of multiple endocrine neoplasia (MEN). [provided by RefSeq, Jul 2008]
• Synonyms: CGI-123; SID1669

Reference Data

• Protein Families: Druggable Genome