GDI1 Mouse Monoclonal Antibody [Clone ID: OTI1B1]

CAT#: CF810977

Carrier-free (BSA/glycerol-free) GDI1 mouse monoclonal antibody,clone OTI1B1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GDI1 (RC203824, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GDI1. Positive lysates LY419896 (100ug) and LC419896 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 3 cell lines by using anti-GDI1 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-GDI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810977) (1:10000)

Specifications

Product Data

• Clone Name: OTI1B1
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:10000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GDI1 (NP_001484) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.4 kDa
• Gene Name: Homo sapiens GDP dissociation inhibitor 1 (GDI1), mRNA.
• Database Link:
  NP_001484
  Entrez Gene 14567 MouseEntrez Gene 25183 RatEntrez Gene 2664 Human
  UniProt ID P31150
• Background: GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI1 is expressed primarily in neural and sensory tissues. Mutations in GDI1 have been linked to X-linked nonspecific mental retardation. [provided by RefSeq, Jul 2008]
• Synonyms: 1A; GDIL; MRX41; MRX48; OPHN2; RABGD1A; RABGDIA; XAP-4

Reference Data

• Protein Families: Druggable Genome