DUSP5 Mouse Monoclonal Antibody [Clone ID: OTI8E12]

CAT#: CF811008

Carrier-free (BSA/glycerol-free) DUSP5 mouse monoclonal antibody,clone OTI8E12

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DUSP5 (RC209128, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DUSP5 (1:2000). Positive lysates LY417998 (100ug) and LC417998 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI8E12
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 125-384 of human DUSP5 (NP_004410) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.9 kDa
• Gene Name: Homo sapiens dual specificity phosphatase 5 (DUSP5), mRNA.
• Database Link:
  NP_004410
  Entrez Gene 171109 RatEntrez Gene 240672 MouseEntrez Gene 1847 Human
  UniProt ID Q16690
• Background: The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, is expressed in a variety of tissues with the highest levels in pancreas and brain, and is localized in the nucleus. [provided by RefSeq, Jul 2008]
• Synonyms: DUSP; HVH3

Reference Data

• Protein Families: Phosphatase
• Protein Pathways: MAPK signaling pathway