Sumo 1 (SUMO1) Mouse Monoclonal Antibody [Clone ID: OTI1E8]

CAT#: CF811055

Carrier-free (BSA/glycerol-free) SUMO1 mouse monoclonal antibody,clone OTI1E8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SUMO1 (RC221677, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SUMO1. Positive lysates LY423653 (100ug) and LC423653 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1E8
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SUMO1 (NP_001005781) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 11.4 kDa
• Gene Name: Homo sapiens small ubiquitin like modifier 1 (SUMO1), transcript variant 2, mRNA.
• Database Link:
  NP_001005781
  Entrez Gene 22218 MouseEntrez Gene 301442 RatEntrez Gene 7341 Human
  UniProt ID P63165
• Background: This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Jul 2008]
• Synonyms: DAP1; GMP1; OFC10; PIC1; SENP2; SMT3; SMT3C; SMT3H3; UBL1

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors