RAX Mouse Monoclonal Antibody [Clone ID: OTI6C2]

CAT#: CF811068

Carrier-free (BSA/glycerol-free) RAX mouse monoclonal antibody,clone OTI6C2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAX (RC219896, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAX. (1:2. Positive lysates LY415546 (100ug) and LC415546 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI6C2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-222 of human RAX (NP_038463) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.5 kDa
• Gene Name: Homo sapiens retina and anterior neural fold homeobox (RAX), mRNA.
• Database Link:
  NP_038463
  Entrez Gene 30062 Human
  UniProt ID Q9Y2V3
• Background: This gene encodes a homeobox-containing transcription factor that functions in eye development. The gene is expressed early in the eye primordia, and is required for retinal cell fate determination and also regulates stem cell proliferation. Mutations in this gene have been reported in patients with defects in ocular development, including microphthalmia, anophthalmia, and coloboma. [provided by RefSeq, Oct 2009]
• Synonyms: MCOP3; RX

Reference Data

• Protein Families: Druggable Genome