CNPase (CNP) Mouse Monoclonal Antibody [Clone ID: OTI3C3]

CAT#: CF811100

Carrier-free (BSA/glycerol-free) CNP mouse monoclonal antibody,clone OTI3C3

Product Images

Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and CNP-Knockout hela cells (KO, Cat# LC810047) were separated by SDS-PAGE and immunoblotted with anti-CNP monoclonal antibody TA811100. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNP (RC207038, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNP (1:2000). Positive lysates LY409699 (100ug) and LC409699 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-CNP monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-CNP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811100) (1:500)

Specifications

Product Data

• Clone Name: OTI3C3
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombiant protein of human CNP (NP_149124) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 47.4 kDa
• Gene Name: Homo sapiens 2',3'-cyclic nucleotide 3' phosphodiesterase (CNP), transcript variant 1, mRNA.
• Database Link:
  NP_149124
  Entrez Gene 12799 MouseEntrez Gene 25275 RatEntrez Gene 1267 Human
  UniProt ID P09543
• Synonyms: CNP1