CCDC22 Mouse Monoclonal Antibody [Clone ID: OTI2E4]

CAT#: CF811111

Carrier-free (BSA/glycerol-free) CCDC22 mouse monoclonal antibody,clone OTI2E4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCDC22 (RC201585, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCDC22 (1:2000). Positive lysates LY415538 (100ug) and LC415538 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 293T cell line by using anti-CCDC22 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CCDC22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811111) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CCDC22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811111) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CCDC22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811111) (1:500)

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Specifications

Product Data

• Clone Name: OTI2E4
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombiant protein of human CCDC22 (NP_054727) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 70.6 kDa
• Gene Name: Homo sapiens coiled-coil domain containing 22 (CCDC22), mRNA.
• Database Link:
  NP_054727
  Entrez Gene 54638 MouseEntrez Gene 317381 RatEntrez Gene 28952 Human
  UniProt ID O60826
• Synonyms: CXorf37; JM1; RTSC2