CCDC22 Mouse Monoclonal Antibody [Clone ID: OTI1B4]

CAT#: CF811119

Carrier-free (BSA/glycerol-free) CCDC22 mouse monoclonal antibody,clone OTI1B4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCDC22 (RC201585, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCDC2. Positive lysates LY415538 (100ug) and LC415538 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1B4
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CCDC22 (NP_054727) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 70.6 kDa
• Gene Name: Homo sapiens coiled-coil domain containing 22 (CCDC22), mRNA.
• Database Link:
  NP_054727
  Entrez Gene 54638 MouseEntrez Gene 317381 RatEntrez Gene 28952 Human
  UniProt ID O60826
• Background: This gene encodes a protein containing a coiled-coil domain. The encoded protein functions in the regulation of NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells) by interacting with COMMD (copper metabolism Murr1 domain-containing) proteins. The mouse orthologous protein has been shown to bind copines, which are calcium-dependent, membrane-binding proteins that may function in calcium signaling. This human gene has been identified as a novel candidate gene for syndromic X-linked intellectual disability. [provided by RefSeq, Aug 2013]
• Synonyms: CXorf37; JM1; RTSC2