MLC1SA (MYL6B) Mouse Monoclonal Antibody [Clone ID: OTI7F10]

CAT#: CF811146

Carrier-free (BSA/glycerol-free) MYL6B mouse monoclonal antibody,clone OTI7F10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYL6B (RC202076, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYL6B (1:2000). Positive lysates LY419310 (100ug) and LC419310 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from K562 cell line and mouse testis tissue lysate by using anti-MYL6B monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and MYL6B-Knockout 293T cells (KO, Cat# LC812315) were separated by SDS-PAGE and immunoblotted with anti-MYL6B monoclonal antibody TA811146, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

Specifications

Product Data

• Clone Name: OTI7F10
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombiant protein of human MYL6B (NP_002466) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22.6 kDa
• Gene Name: Homo sapiens myosin light chain 6B (MYL6B), transcript variant 2, mRNA.
• Database Link:
  NP_002466
  Entrez Gene 216459 MouseEntrez Gene 140465 Human
  UniProt ID P14649
• Synonyms: MLC1SA

Reference Data

• Protein Pathways: Vascular smooth muscle contraction