SF3B14 (SF3B6) Mouse Monoclonal Antibody [Clone ID: OTI8A1]

CAT#: CF811148

Carrier-free (BSA/glycerol-free) SF3B14 mouse monoclonal antibody,clone OTI8A1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SF3B14 (RC201866, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SF3B14 (1:2000). Positive lysates LY414228 (100ug) and LC414228 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SF3B14 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811148) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-SF3B14 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811148) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SF3B14 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811148) (1:1000)

Specifications

Product Data

• Clone Name: OTI8A1
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:1000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombiant protein of human SF3B14 (NP_057131) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 14.4 kDa
• Gene Name: Homo sapiens splicing factor 3b subunit 6 (SF3B6), mRNA.
• Database Link:
  NP_057131
  Entrez Gene 66055 MouseEntrez Gene 100365504 RatEntrez Gene 51639 Human
  UniProt ID Q9Y3B4
• Synonyms: CGI-110; HSPC175; Ht006; P14; SAP14; SAP14a; SF3B14; SF3B14a

Reference Data

• Protein Pathways: Spliceosome