HHLA3 Mouse Monoclonal Antibody [Clone ID: OTI4E10]

CAT#: CF811171

Carrier-free (BSA/glycerol-free) HHLA3 mouse monoclonal antibody,clone OTI4E10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HHLA3 (RC213042, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HHLA3 (1:2000). Positive lysates LY421904 (100ug) and LC421904 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4E10
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombiant protein of human HHLA3 (NP_001026863) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 13.3 kDa
• Gene Name: Homo sapiens HERV-H LTR-associating 3 (HHLA3), transcript variant 1, mRNA.
• Database Link:
  NP_001026863
  Entrez Gene 11147 Human
  UniProt ID Q9XRX5
• Synonyms: HERV-H LTR-associating 3; human endogenous retrovirus-H long terminal repeat-associating 3; OTTHUMP00000010943