AIF (AIFM1) Mouse Monoclonal Antibody [Clone ID: OTI8B8]

CAT#: CF811180

Carrier-free (BSA/glycerol-free) AIFM1 mouse monoclonal antibody,clone OTI8B8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AIFM1 (RC224064, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AIFM1 (1:2000).

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  Western blot analysis of extracts (35ug) from 6 different cell lines and mouse liver tissue lysate by using anti-AIFM1 monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and AIFM1-Knockout 293T cells (KO, Cat# LC811853) were separated by SDS-PAGE and immunoblotted with anti-AIFM1 monoclonal antibody TA811180, (1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control.

Specifications

Product Data

• Clone Name: OTI8B8
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 134-483 of human AIFM1 (NP_001124318) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens apoptosis inducing factor mitochondria associated 1 (AIFM1), transcript variant 4, mRNA.
• Database Link:
  NP_001124318
  Entrez Gene 26926 MouseEntrez Gene 83533 RatEntrez Gene 9131 Human
  UniProt ID O95831
• Synonyms: AIF; CMT2D; CMTX4; COWCK; COXPD6; NADMR; NAMSD; PDCD8

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Apoptosis