AMCase (CHIA) Mouse Monoclonal Antibody [Clone ID: OTI3H4]

CAT#: CF811183

Carrier-free (BSA/glycerol-free) CHIA mouse monoclonal antibody,clone OTI3H4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHIA (RC206441, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHIA (1:2000). Positive lysates LY411918 (100ug) and LC411918 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-CHIA monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CHIA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811183) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human gastric tissue within the normal limits using anti-CHIA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811183) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-CHIA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811183) (1:2000)

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Specifications

Product Data

• Clone Name: OTI3H4
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human Recombinant protein of human CHIA (NP_068569) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens chitinase acidic (CHIA), transcript variant 2, mRNA.
• Database Link:
  NP_068569
  Entrez Gene 27159 Human
  UniProt ID Q9BZP6
• Background: The protein encoded by this gene degrades chitin, which is found in the cell wall of most fungi as well as in arthropods and some nematodes. The encoded protein can also stimulate interleukin 13 expression, and variations in this gene can lead to asthma susceptibility. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
• Synonyms: AMCASE; CHIT2; TSA1902

Reference Data

• Protein Families: Secreted Protein
• Protein Pathways: Amino sugar and nucleotide sugar metabolism