TBX3 Mouse Monoclonal Antibody [Clone ID: OTI8H5]

CAT#: CF811202

Carrier-free (BSA/glycerol-free) TBX3 mouse monoclonal antibody,clone OTI8H5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBX3 (RC206829, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TBX3. Positive lysates LY413892 (100ug) and LC413892 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI8H5
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 244-481 of human TBX3 (NP_057653) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 79.2 kDa
• Gene Name: Homo sapiens T-box transcription factor 3 (TBX3), transcript variant 2, mRNA.
• Database Link:
  NP_057653
  Entrez Gene 21386 MouseEntrez Gene 353305 RatEntrez Gene 6926 Human
  UniProt ID O15119
• Background: This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This protein is a transcriptional repressor and is thought to play a role in the anterior/posterior axis of the tetrapod forelimb. Mutations in this gene cause ulnar-mammary syndrome, affecting limb, apocrine gland, tooth, hair, and genital development. Alternative splicing of this gene results in three transcript variants encoding different isoforms; however, the full length nature of one variant has not been determined. [provided by RefSeq, Jul 2008]
• Synonyms: TBX3-ISO; UMS; XHL

Reference Data

• Protein Families: Druggable Genome, Transcription Factors