RNF4 Mouse Monoclonal Antibody [Clone ID: OTI1F6]

CAT#: CF811231

Carrier-free (BSA/glycerol-free) RNF4 mouse monoclonal antibody,clone OTI1F6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF4 (RC207273, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF4 (1:2000). Positive lysates LY432659 (100ug) and LC432659 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 5 different cell lines by using anti-RNF4 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI1F6
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-190 of human RNF4 (NP_002929) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens ring finger protein 4 (RNF4), transcript variant 1, mRNA.
• Database Link:
  NP_001171938
  Entrez Gene 19822 MouseEntrez Gene 29274 RatEntrez Gene 6047 Human
  UniProt ID P78317
• Background: The protein encoded by this gene contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent transcription mediated by this protein. Studies of the mouse and rat counterparts suggested a role of this protein in spermatogenesis. A pseudogene of this gene is found on chromosome 1. [provided by RefSeq, Jul 2010]
• Synonyms: RES4-26; SLX5; SNURF

Reference Data

• Protein Families: Transcription Factors