PPAR delta (PPARD) Mouse Monoclonal Antibody [Clone ID: OTI4D10]

CAT#: CF811238

Carrier-free (BSA/glycerol-free) PPARD mouse monoclonal antibody,clone OTI4D10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPARD (RC214735, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPARD (1:2000). Positive lysates LY416782 (100ug) and LC416782 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human adult brain tissue within the normal limits using anti-PPARD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811238) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human placenta tissue within the normal limits using anti-PPARD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811238) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-PPARD mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA (1:150)

Specifications

Product Data

• Clone Name: OTI4D10
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-244 of human PPARD (NP_006229) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.7 kDa
• Gene Name: Homo sapiens peroxisome proliferator activated receptor delta (PPARD), transcript variant 1, mRNA.
• Database Link:
  NP_006229
  Entrez Gene 19015 MouseEntrez Gene 25682 RatEntrez Gene 5467 Human
  UniProt ID Q03181
• Synonyms: FAAR; NR1C2; NUC1; NUCI; NUCII; PPARB

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer, PPAR signaling pathway, Wnt signaling pathway