RGS17 Mouse Monoclonal Antibody [Clone ID: OTI9G4]

CAT#: CF811255

Carrier-free (BSA/glycerol-free) RGS17 mouse monoclonal antibody,clone OTI9G4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGS17 (RC201859, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGS17 (1:2000). Positive lysates LY402209 (100ug) and LC402209 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI9G4
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RGS17 (NP_036551) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 24.2 kDa
• Gene Name: Homo sapiens regulator of G protein signaling 17 (RGS17), mRNA.
• Database Link:
  NP_036551
  Entrez Gene 26575 Human
  UniProt ID Q9UGC6
• Background: This gene encodes a member of the regulator of G-protein signaling family. This protein contains a conserved, 120 amino acid motif called the RGS domain and a cysteine-rich region. The protein attenuates the signaling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. [provided by RefSeq, Jul 2008]
• Synonyms: hRGS17; RGS-17; RGSZ2

Reference Data

• Protein Families: Druggable Genome