Paxillin (PXN) Mouse Monoclonal Antibody [Clone ID: OTI6B7]

CAT#: CF811264

Carrier-free (BSA/glycerol-free) PXN mouse monoclonal antibody,clone OTI6B7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PXN (RC225692, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PXN (1:2000). Positive lysates LY429750 (100ug) and LC429750 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI6B7
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombiant protein of human PXN (NP_079433) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.4 kDa
• Gene Name: Homo sapiens paxillin (PXN), transcript variant 4, mRNA.
• Database Link:
  NP_079433
  Entrez Gene 19303 MouseEntrez Gene 360820 RatEntrez Gene 5829 Human
  UniProt ID P49023
• Synonyms: FLJ16691

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, VEGF signaling pathway