Septin 3 (SEPT3) Mouse Monoclonal Antibody [Clone ID: OTI2G3]

CAT#: CF811272

Carrier-free (BSA/glycerol-free) SEPT3 mouse monoclonal antibody,clone OTI2G3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEPT3 (RC215771, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEPT3 (1:2000). Positive lysates LY403438 (100ug) and LC403438 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from mouse brain tissue lysate by using anti-SEPT3 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human adult brain tissue within the normal limits using anti-SEPT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811272) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-SEPT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811272) (1:500)

Specifications

Product Data

• Clone Name: OTI2G3
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombiant protein of human SEPT3 (NP_663786) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.5 kDa
• Gene Name: Homo sapiens septin 3 (SEPTIN3), transcript variant A, mRNA.
• Database Link:
  NP_663786
  Entrez Gene 24050 MouseEntrez Gene 55964 Human
  UniProt ID Q9UH03
• Synonyms: bK250D10.3; SEP3