PYGM Mouse Monoclonal Antibody [Clone ID: OTI3F9]

CAT#: CF811315

Carrier-free (BSA/glycerol-free) PYGM mouse monoclonal antibody,clone OTI3F9

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYGM (RC212365, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYGM (1:2000). Positive lysates LY401719 (100ug) and LC401719 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human adult heart tissue within the normal limits using anti-PYGM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811315) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human muscle tissue within the normal limits using anti-PYGM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811315) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human gastric stromal tumor tissue using anti-PYGM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811315) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human testicle tissue within the normal limits using anti-PYGM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811315) (1:2000)

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Specifications

Product Data

• Clone Name: OTI3F9
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 698-842 of human PYGM (NP_005600) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens glycogen phosphorylase, muscle associated (PYGM), transcript variant 1, mRNA.
• Database Link:
  NP_005600
  Entrez Gene 19309 MouseEntrez Gene 24701 RatEntrez Gene 5837 Human
  UniProt ID P11217
• Background: This gene encodes a muscle enzyme involved in glycogenolysis. Highly similar enzymes encoded by different genes are found in liver and brain. Mutations in this gene are associated with McArdle disease (myophosphorylase deficiency), a glycogen storage disease of muscle. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
• Synonyms: glycogen; glycogen phosphorylase; glycogen storage disease type V; glycogen storage disease type V); McArdle syndrome; muscle; muscle (McArdle syndrome; myophosphorylase; phosphorylase

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Insulin signaling pathway, Starch and sucrose metabolism