C16orf57 (USB1) Mouse Monoclonal Antibody [Clone ID: OTI1B1]

CAT#: CF811328

Carrier-free (BSA/glycerol-free) C16orf57 mouse monoclonal antibody,clone OTI1B1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C16orf57 (RC202591, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C16orf57 (1:2000). Positive lysates LY411214 (100ug) and LC411214 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 5 different cell lines by using anti-C16orf57 monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and USB1-Knockout HeLa cells (KO, Cat# LC832371) were separated by SDS-PAGE and immunoblotted with anti-USB1 monoclonal antibody TA811328 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

Specifications

Product Data

• Clone Name: OTI1B1
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human C16orf57 (NP_078874) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.1 kDa
• Gene Name: Homo sapiens U6 snRNA biogenesis phosphodiesterase 1 (USB1), transcript variant 1, mRNA.
• Database Link:
  NP_078874
  Entrez Gene 79650 Human
  UniProt ID Q9BQ65
• Background: This gene encodes a protein with several conserved domains, however, its exact function is not known. Mutations in this gene are associated with poikiloderma with neutropenia (PN), which shows phenotypic overlap with Rothmund-Thomson syndrome (RTS) caused by mutations in the RECQL4 gene. It is believed that this gene product interacts with RECQL4 protein via SMAD4 proteins, explaining the partial clinical overlap between PN and RTS. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011]
• Synonyms: C16orf57; hUsb1; HVSL1; Mpn1; PN