CSPS (SULT1A3) Mouse Monoclonal Antibody [Clone ID: OTI1C6]

CAT#: CF811332

Carrier-free (BSA/glycerol-free) SULT1A3 mouse monoclonal antibody,clone OTI1C6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SULT1A3 (RC218162, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SULT1A3 (1:2000). Positive lysates LY430480 (100ug) and LC430480 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from HepG2 cell line by using anti-SULT1A3 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SULT1A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811332) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SULT1A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811332) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-SULT1A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811332) (1:500)

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Specifications

Product Data

• Clone Name: OTI1C6
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombiant protein of human SULT1A3 (NP_808220) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34 kDa
• Gene Name: Homo sapiens sulfotransferase family 1A member 3 (SULT1A3), mRNA.
• Database Link:
  NP_808220
  Entrez Gene 6818 Human
• Synonyms: HAST; HAST3; M-PST; ST1A3; ST1A4; ST1A5; STM; TL-PST

Reference Data

• Protein Pathways: Sulfur metabolism