Miz1 (ZBTB17) Mouse Monoclonal Antibody [Clone ID: OTI7G8]

CAT#: CF811358

Carrier-free (BSA/glycerol-free) ZBTB17 mouse monoclonal antibody,clone OTI7G8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZBTB17 (RC216143, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZBTB17 (1:2000). Positive lysates LY401167 (100ug) and LC401167 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI7G8
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 112-457 of human ZBTB17 (NP_003434) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens zinc finger and BTB domain containing 17 (ZBTB17), transcript variant 2, mRNA.
• Database Link:
  NP_003434
  Entrez Gene 7709 Human
  UniProt ID Q13105
• Background: This gene encodes a zinc finger protein involved in the regulation of c-myc. The symbol MIZ1 has also been associated with PIAS2 which is a different gene located on chromosome 18. [provided by RefSeq, Jul 2008]
• Synonyms: MIZ-1; pHZ-67; ZNF60; ZNF151

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Cell cycle