TXNRD1 Mouse Monoclonal Antibody [Clone ID: OTI7B2]

CAT#: CF811366

Carrier-free (BSA/glycerol-free) TXNRD1 mouse monoclonal antibody,clone OTI7B2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TXNRD1 (RC202040, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TXNRD1 (1:2000).

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  Western blot analysis of extracts (35ug) from 3 different cell lines and 2 different tissue lysates by using anti-TXNRD1 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TXNRD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811366) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TXNRD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811366) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TXNRD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811366) (1:2000)

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Specifications

Product Data

• Clone Name: OTI7B2
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TXNRD1 (NP_003321) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens thioredoxin reductase 1 (TXNRD1), transcript variant 4, mRNA.
• Database Link:
  NP_003321
  Entrez Gene 50493 MouseEntrez Gene 58819 RatEntrez Gene 7296 Human
  UniProt ID Q16881
• Synonyms: GRIM-12; TR; TR1; TRXR1; TXNR

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Pyrimidine metabolism