VRK2 Mouse Monoclonal Antibody [Clone ID: OTI2A2]

CAT#: CF811396

Carrier-free (BSA/glycerol-free) VRK2 mouse monoclonal antibody,clone OTI2A2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VRK2 (RC206522, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VRK2 (1:2000). Positive lysates LY416739 (100ug) and LC416739 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2A2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 301-486 of human VRK2 (NP_006287) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens VRK serine/threonine kinase 2 (VRK2), transcript variant 1, mRNA.
• Database Link:
  NP_006287
  Entrez Gene 7444 Human
  UniProt ID Q86Y07
• Background: This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. The encoded protein acts as an effector of signaling pathways that regulate apoptosis and tumor cell growth. Variants in this gene have been associated with schizophrenia. Alternative splicing results in multiple transcript variants that differ in their subcellular localization and biological activity. [provided by RefSeq, Jan 2014]
• Synonyms: vaccinia-related kinase-2; vaccinia related kinase 2; vaccinia virus B1R-related kinase 2

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane