TSPYL1 Mouse Monoclonal Antibody [Clone ID: OTI6C6]

CAT#: CF811400

Carrier-free (BSA/glycerol-free) TSPYL1 mouse monoclonal antibody,clone OTI6C6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSPYL1 (RC208456, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSPYL. Positive lysates LY418771 (100ug) and LC418771 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI6C6
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-272 of human TSPYL1 (NP_003300) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49 kDa
• Gene Name: Homo sapiens TSPY like 1 (TSPYL1), mRNA.
• Database Link:
  NP_003300
  Entrez Gene 7259 Human
  UniProt ID Q9H0U9
• Background: The protein encoded by this gene is found in the nucleolus and is similar to that of a family of genes on the Y-chromosome. This gene is intronless. Defects in this gene are a cause of sudden infant death with dysgenesis of the testes syndrome (SIDDT). [provided by RefSeq, Dec 2009]
• Synonyms: TSPYL