LIMS2 Mouse Monoclonal Antibody [Clone ID: OTI5H8]

CAT#: CF811408

Carrier-free (BSA/glycerol-free) LIMS2 mouse monoclonal antibody,clone OTI5H8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIMS2 (RC213746, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIMS2 (1:2000). Positive lysates LY413411 (100ug) and LC413411 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from mouse liver tissue lysate by using anti-LIMS2 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI5H8
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-363 of human LIMS2 (NP_060450) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.3 kDa
• Gene Name: Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant 2, mRNA.
• Database Link:
  NP_060450
  Entrez Gene 225341 MouseEntrez Gene 361303 RatEntrez Gene 55679 Human
  UniProt ID Q7Z4I7
• Synonyms: LGMD2W; PINCH-2; PINCH2