SIX3 Mouse Monoclonal Antibody [Clone ID: OTI7G9]

CAT#: CF811410

Carrier-free (BSA/glycerol-free) SIX3 mouse monoclonal antibody,clone OTI7G9

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIX3 (RC216286, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIX3 (1:2000). Positive lysates LY401662 (100ug) and LC401662 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI7G9
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 142-332 of human SIX3 (NP_005404) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens SIX homeobox 3 (SIX3), mRNA.
• Database Link:
  NP_005404
  Entrez Gene 20473 MouseEntrez Gene 78974 RatEntrez Gene 6496 Human
  UniProt ID O95343
• Background: This gene encodes a member of the sine oculis homeobox transcription factor family. The encoded protein plays a role in eye development. Mutations in this gene have been associated with holoprosencephaly type 2. [provided by RefSeq, Oct 2009]
• Synonyms: HPE2

Reference Data

• Protein Families: Druggable Genome