PPP2R1B Mouse Monoclonal Antibody [Clone ID: OTI8B6]

CAT#: CF811413

Carrier-free (BSA/glycerol-free) PPP2R1B mouse monoclonal antibody,clone OTI8B6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP2R1B (RC230265, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP2R1B (1:2000). Positive lysates LY433265 (100ug) and LC433265 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-PPP2R1B monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and PPP2R1B-Knockout 293T cells (KO, Cat# LC811012) were separated by SDS-PAGE and immunoblotted with anti-PPP2R1B monoclonal antibody TA811413, (1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PPP2R1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811413) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PPP2R1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811413) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PPP2R1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811413) (1:500)

• Immunohistochemical staining of paraffin-embedded Human gastric stromal tumor tissue using anti-PPP2R1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811413) (1:500)

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Specifications

Product Data

• Clone Name: OTI8B6
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 19-601 of human PPP2R1B (NP_859050) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens protein phosphatase 2 scaffold subunit Abeta (PPP2R1B), transcript variant 4, mRNA.
• Database Link:
  NP_001171033
  Entrez Gene 73699 MouseEntrez Gene 315648 RatEntrez Gene 5519 Human
  UniProt ID P30154
• Synonyms: PP2A-Abeta; PR65B

Reference Data

• Protein Families: Druggable Genome, Phosphatase, Transcription Factors
• Protein Pathways: Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway