Trefoil Factor 3 (TFF3) Mouse Monoclonal Antibody [Clone ID: OTI3G5]

CAT#: CF811435

Carrier-free (BSA/glycerol-free) TFF3 mouse monoclonal antibody,clone OTI3G5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFF3 (RC223558, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TFF3. Positive lysates LY418823 (100ug) and LC418823 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3G5
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 72-130 of human TFF3 (NP_003217) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 14.25 kDa
• Gene Name: Homo sapiens trefoil factor 3 (TFF3), mRNA.
• Database Link:
  NP_003217
  Entrez Gene 7033 Human
  UniProt ID Q07654
• Background: Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]
• Synonyms: ITF; P1B; TFI

Reference Data

• Protein Families: Secreted Protein