RUSC2 Mouse Monoclonal Antibody [Clone ID: OTI1A1]

CAT#: CF811460

Carrier-free (BSA/glycerol-free) RUSC2 mouse monoclonal antibody,clone OTI1A1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RUSC2 (RC215610, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RUSC2 (1:2000). Positive lysates LY415015 (100ug) and LC415015 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1A1
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1176-1516 of human RUSC2 (NP_055621) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens RUN and SH3 domain containing 2 (RUSC2), transcript variant 2, mRNA.
• Database Link:
  NP_055621
  Entrez Gene 100213 MouseEntrez Gene 9853 Human
  UniProt ID Q8N2Y8
• Background: This gene encodes a RUN and SH3 domain containing protein that interacts with Rab1b and Rab1-binding protein GM130. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jun 2012]
• Synonyms: Iporin