NDUFA2 Mouse Monoclonal Antibody [Clone ID: OTI5A5]

CAT#: CF811465

Carrier-free (BSA/glycerol-free) NDUFA2 mouse monoclonal antibody,clone OTI5A5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFA2 (RC202715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFA2 (1:200). Positive lysates LY419296 (100ug) and LC419296 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human gastric tissue within the normal limits using anti-NDUFA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811465) (1:150)

Specifications

Product Data

• Clone Name: OTI5A5
• Applications: IHC, WB
• Recommended Dilution: IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 2-99 of human NDUFA2 (NP_002479) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 10.7 kDa
• Gene Name: Homo sapiens NADH:ubiquinone oxidoreductase subunit A2 (NDUFA2), transcript variant 1, mRNA.
• Database Link:
  NP_002479
  Entrez Gene 4695 Human
  UniProt ID O43678
• Background: The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox processes. Mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
• Synonyms: B8; CD14; CIB8

Reference Data

• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease