Peroxiredoxin 5 (PRDX5) Mouse Monoclonal Antibody [Clone ID: OTI3B6]

CAT#: CF811474

Carrier-free (BSA/glycerol-free) PRDX5 mouse monoclonal antibody,clone OTI3B6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRDX5 (RC210709, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRDX5 (1:2000). Positive lysates LY402144 (100ug) and LC402144 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-PRDX5 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human gastric tissue within the normal limits using anti-PRDX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811474) (1:250)

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  Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-PRDX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811474) (1:250)

Specifications

Product Data

• Clone Name: OTI3B6
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:250
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PRDX5 (NP_036226) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens peroxiredoxin 5 (PRDX5), transcript variant 1, mRNA.
• Database Link:
  NP_036226
  Entrez Gene 54683 MouseEntrez Gene 113898 RatEntrez Gene 25824 Human
  UniProt ID P30044
• Background: This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. This gene uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: ACR1; AOEB166; B166; HEL-S-55; PLP; PMP20; PRDX6; prx-V; PRXV; SBBI10

Reference Data

• Protein Families: Druggable Genome