KLC2 Mouse Monoclonal Antibody [Clone ID: OTI10H10]

CAT#: CF811478

Carrier-free (BSA/glycerol-free) KLC2 mouse monoclonal antibody,clone OTI10H10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLC2 (RC214206, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLC2 (1:2000). Positive lysates LY402950 (100ug) and LC402950 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from HELA cell line by using anti-KLC2 monoclonal antibody (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-KLC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811478) (1:2000)

• 

  Immunohistochemical staining of paraffin-embedded Human testicle tissue within the normal limits using anti-KLC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811478) (1:2000)

Specifications

Product Data

• Clone Name: OTI10H10
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 335-622 of human KLC2 (NP_073733) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens kinesin light chain 2 (KLC2), transcript variant 1, mRNA.
• Database Link:
  NP_073733
  Entrez Gene 64837 Human
  UniProt ID Q9H0B6
• Background: Kinesin is a molecular motor that generates ATP-dependent movement of vesicles and organelles along microtubules. Kinesin consists of 2 light chains, such as KLC2, and 2 heavy chains (see KIF5B; MIM 602809) in a 1:1 stoichiometric ratio (Rahman et al., 1998 [PubMed 9624122]). [supplied by OMIM, Mar 2008]
• Synonyms: SPOAN

Reference Data

• Protein Families: Druggable Genome