IMP3 (IGF2BP3) Mouse Monoclonal Antibody [Clone ID: OTI6A3]

CAT#: CF811496

Carrier-free (BSA/glycerol-free) IGF2BP3 mouse monoclonal antibody,clone OTI6A3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP3 (RC209597, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP3 (1:2000). Positive lysates LY416563 (100ug) and LC416563 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-IGF2BP3 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-IGF2BP3 mouse monoclonal antibody. This figure shows negative staining. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811496) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human testicle tissue within the normal limits using anti-IGF2BP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811496) (1:150)

Specifications

Product Data

• Clone Name: OTI6A3
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human IGF2BP3 (NP_006538) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens insulin like growth factor 2 mRNA binding protein 3 (IGF2BP3), mRNA.
• Database Link:
  NP_006538
  Entrez Gene 140488 MouseEntrez Gene 312320 RatEntrez Gene 10643 Human
  UniProt ID O00425
• Background: The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes. [provided by RefSeq, Jul 2008]
• Synonyms: CT98; IMP-3; IMP3; KOC; KOC1; VICKZ3