WIPI1 Mouse Monoclonal Antibody [Clone ID: OTI11B11]

CAT#: CF811510

Carrier-free (BSA/glycerol-free) WIPI1 mouse monoclonal antibody,clone OTI11B11

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WIPI1 (RC208094, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WIPI1 (1:2000). Positive lysates LY402635 (100ug) and LC402635 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-WIPI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811510) (1:500)

Specifications

Product Data

• Clone Name: OTI11B11
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 195-446 of human WIPI1 (NP_060453) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 48.5 kDa
• Gene Name: Homo sapiens WD repeat domain, phosphoinositide interacting 1 (WIPI1), transcript variant 1, mRNA.
• Database Link:
  NP_060453
  Entrez Gene 52639 MouseEntrez Gene 303630 RatEntrez Gene 55062 Human
  UniProt ID Q5MNZ9
• Background: This gene encodes a WD40 repeat protein. Members of the WD40 repeat family are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
• Synonyms: ATG18; ATG18A; WIPI49