CD14 Mouse Monoclonal Antibody [Clone ID: OTI12C8]

CAT#: CF811528

Carrier-free (BSA/glycerol-free) CD14 mouse monoclonal antibody,clone OTI12C8

Product Images

Western blot analysis of extracts (35ug) from PBMC by using anti-CD14 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD14 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811528) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD14 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811528) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD14 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811528) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human Gastric Carcinoma using anti-CD14 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811528) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-CD14 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811528) (1:2000)

• HEK293T cells transfected with either RC203819 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CD14 antibody (TA811528), and then analyzed by flow cytometry (1:100).

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Specifications

Product Data

• Clone Name: OTI12C8
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 20-367 of human CD14 (NP_000582) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.08 kDa
• Gene Name: Homo sapiens CD14 molecule (CD14), transcript variant 1, mRNA.
• Database Link:
  NP_000582
  Entrez Gene 929 Human
  UniProt ID P08571
• Background: The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]
• Synonyms: CD14 antigen; CD14 molecule; monocyte differentiation antigen CD14

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Hematopoietic cell lineage, MAPK signaling pathway, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Toll-like receptor signaling pathway