FAM119A (METTL21A) Mouse Monoclonal Antibody [Clone ID: OTI3D11]

CAT#: CF811541

Carrier-free (BSA/glycerol-free) METTL21A mouse monoclonal antibody,clone OTI3D11

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY METTL21A (RC225260, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-METTL21A (1:2000). Positive lysates LY426777 (100ug) and LC426777 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3D11
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human METTL21A (NP_001120867) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 24.4 kDa
• Gene Name: Homo sapiens methyltransferase like 21A (METTL21A), transcript variant 2, mRNA.
• Database Link:
  NP_001120867
  Entrez Gene 67099 MouseEntrez Gene 102553119 RatEntrez Gene 151194 Human
  UniProt ID Q8WXB1
• Synonyms: FAM119A; HCA557b; HSPA-KMT