HOXA3 Mouse Monoclonal Antibody [Clone ID: OTI1A1]

CAT#: CF811550

Carrier-free (BSA/glycerol-free) HOXA3 mouse monoclonal antibody,clone OTI1A1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HOXA3 (RC202355, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HOXA3 (1:2000). Positive lysates LY410755 (100ug) and LC410755 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1A1
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 40-276 of human HOXA3 (NP_109377) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens homeobox A3 (HOXA3), transcript variant 1, mRNA.
• Database Link:
  NP_109377
  Entrez Gene 15400 MouseEntrez Gene 103690130 RatEntrez Gene 3200 Human
  UniProt ID O43365
• Background: In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: HOX1; HOX1E

Reference Data

• Protein Families: Transcription Factors