Repulsive Guidance Molecule A (RGMA) Mouse Monoclonal Antibody [Clone ID: OTI4G8]

CAT#: CF811560

Carrier-free (BSA/glycerol-free) RGMA mouse monoclonal antibody,clone OTI4G8

Product Images

Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-RGMA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811560) (1:250)

Specifications

Product Data

• Clone Name: OTI4G8
• Applications: IHC
• Recommended Dilution: IHC 1:250
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 169-424 of human RGMA (NP_064596) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.2 kDa
• Gene Name: Homo sapiens repulsive guidance molecule BMP co-receptor a (RGMA), transcript variant 4, mRNA.
• Database Link:
  NP_064596
  Entrez Gene 244058 MouseEntrez Gene 308739 RatEntrez Gene 56963 Human
  UniProt ID Q96B86
• Synonyms: RGM