REV1 Mouse Monoclonal Antibody [Clone ID: OTI1E12]

CAT#: CF811575

Carrier-free (BSA/glycerol-free) REV1 mouse monoclonal antibody,clone OTI1E12

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY REV1 (RC221742, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-REV1 (1:2000). Positive lysates LY402538 (100ug) and LC402538 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-REV1 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI1E12
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1079-1251 of human REV1 (NP_057400) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens REV1 DNA directed polymerase (REV1), transcript variant 1, mRNA.
• Database Link:
  NP_057400
  Entrez Gene 316344 RatEntrez Gene 51455 Human
  UniProt ID Q9UBZ9
• Background: This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. [provided by RefSeq, Mar 2016]
• Synonyms: AIBP80; REV1L

Reference Data

• Protein Families: Druggable Genome