ZFP57 Mouse Monoclonal Antibody [Clone ID: OTI1E2]

CAT#: CF811579

Carrier-free (BSA/glycerol-free) ZFP57 mouse monoclonal antibody,clone OTI1E2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZFP57 (RC229273, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZFP57 (1:2000). Positive lysates LY432292 (100ug) and LC432292 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-ZFP57 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human testicle tissue within the normal limits using anti-ZFP57 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811579) (1:2000)

Specifications

Product Data

• Clone Name: OTI1E2
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ZFP57 (NP_001103279) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens ZFP57 zinc finger protein (ZFP57), transcript variant 1, mRNA.
• Database Link:
  NP_001103279
  Entrez Gene 22715 MouseEntrez Gene 346171 Human
  UniProt ID Q9NU63
• Background: The protein encoded by this gene is a zinc finger protein containing a KRAB domain. Studies in mouse suggest that this protein may function as a transcriptional repressor. Mutations in this gene have been associated with transient neonatal diabetes mellitus type 1 (TNDM1). [provided by RefSeq, Sep 2009]
• Synonyms: bA145L22; bA145L22.2; C6orf40; TNDM1; ZNF698

Reference Data

• Protein Families: ES Cell Differentiation/IPS