LSDP5 (PLIN5) Mouse Monoclonal Antibody [Clone ID: OTI3F2]

CAT#: CF811630

Carrier-free (BSA/glycerol-free) PLIN5 mouse monoclonal antibody,clone OTI3F2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLIN5 (RC224783, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLIN5 (1:2000). Positive lysates LY423024 (100ug) and LC423024 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-PLIN5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811630) (1:500)

Specifications

Product Data

• Clone Name: OTI3F2
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-463 of human PLIN5 (NP_001013728) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51.2 kDa
• Gene Name: Homo sapiens perilipin 5 (PLIN5), mRNA.
• Database Link:
  NP_001013728
  Entrez Gene 440503 Human
  UniProt ID Q00G26
• Background: Members of the perilipin family, such as PLIN5, coat intracellular lipid storage droplets and protect them from lipolytic degradation (Dalen et al., 2007 [PubMed 17234449]). [supplied by OMIM, Feb 2010]
• Synonyms: LSDA5; LSDP5; MLDP; OXPAT