DLX1 Mouse Monoclonal Antibody [Clone ID: OTI1E6]

CAT#: CF811631

Carrier-free (BSA/glycerol-free) DLX1 mouse monoclonal antibody,clone OTI1E6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DLX1 (RC206895, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DLX1 (1:2000). Positive lysates LY406033 (100ug) and LC406033 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human adult brain tissue within the normal limits using anti-DLX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811631) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-DLX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811631) (1:500)

Specifications

Product Data

• Clone Name: OTI1E6
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-255 of human DLX1 (NP_835221) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens distal-less homeobox 1 (DLX1), transcript variant 1, mRNA.
• Database Link:
  NP_835221
  Entrez Gene 13390 MouseEntrez Gene 296500 RatEntrez Gene 1745 Human
  UniProt ID P56177
• Background: This gene encodes a member of a homeobox transcription factor gene family similiar to the Drosophila distal-less gene. The encoded protein is localized to the nucleus where it may function as a transcriptional regulator of signals from multiple TGF-{beta} superfamily members. The encoded protein may play a role in the control of craniofacial patterning and the differentiation and survival of inhibitory neurons in the forebrain. This gene is located in a tail-to-tail configuration with another member of the family on the long arm of chromosome 2. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
• Synonyms: distal-less homeo box 1; distal-less homeobox 1; OTTHUMP00000082494; OTTHUMP00000082497

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transcription Factors