TM4SF2 (TSPAN7) Mouse Monoclonal Antibody [Clone ID: OTI2D6]

CAT#: CF811638

Carrier-free (BSA/glycerol-free) TSPAN7 mouse monoclonal antibody,clone OTI2D6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSPAN7 (RC205248, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSPAN7 (1:500). Positive lysates LY401460 (100ug) and LC401460 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from CCRF-CEM cell line by using anti-TSPAN7 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-TSPAN7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811638) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TSPAN7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811638) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TSPAN7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811638) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TSPAN7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811638) (1:500)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TSPAN7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811638) (1:500)

• Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-TSPAN7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811638) (1:500)

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Specifications

Product Data

• Clone Name: OTI2D6
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TSPAN7 (NP_004606) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens tetraspanin 7 (TSPAN7), mRNA.
• Database Link:
  NP_004606
  Entrez Gene 7102 Human
  UniProt ID P41732
• Background: The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein and may have a role in the control of neurite outgrowth. It is known to complex with integrins. This gene is associated with X-linked mental retardation and neuropsychiatric diseases such as Huntington's chorea, fragile X syndrome and myotonic dystrophy. [provided by RefSeq, Jul 2008]
• Synonyms: A15; CCG-B7; CD231; DXS1692E; MRX58; MXS1; TALLA-1; TM4SF2; TM4SF2b

Reference Data

• Protein Families: Druggable Genome, Transmembrane