TOPBP1 Mouse Monoclonal Antibody [Clone ID: OTI5C12]

CAT#: CF811658

Carrier-free (BSA/glycerol-free) TOPBP1 mouse monoclonal antibody,clone OTI5C12

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOPBP1 (RC218584, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOPBP. Positive lysates LY416246 (100ug) and LC416246 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI5C12
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 892-1146 of human TOPBP1 (NP_008958) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 171.1 kDa
• Gene Name: Homo sapiens DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant 1, mRNA.
• Database Link:
  NP_008958
  Entrez Gene 11073 Human
  UniProt ID Q92547
• Background: This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands. [provided by RefSeq, Jul 2008]
• Synonyms: TOP2BP1