GPR17 Mouse Monoclonal Antibody [Clone ID: OTI6B9]

CAT#: CF811665

Carrier-free (BSA/glycerol-free) GPR17 mouse monoclonal antibody,clone OTI6B9

Product Images

Immunohistochemical staining of paraffin-embedded Human adult brain tissue within the normal limits using anti-GPR17 mouse monoclonal antibody. This figure shows negative staining. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811665) (1:150)

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  Immunohistochemical staining of paraffin-embedded cell pellets of 293T cells transfected with pcDNA3.1-mGPR17, pcDNA3.1-rGPR17 or pcDNA3.1 plasmids using anti-GPR17 Mouse monoclonal antibody (Heat-induced epitope retrieval by Tris-EDTA buffer (pH8.0) at 120°C for 2.5 min) (1:150).

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  Immunohistochemical staining of paraffin-embedded cell pellets of 293T cells transfected with pCINEO-hGPR17 or pCINEO plasmids using anti-GPR17 Mouse monoclonal antibody. This figure shows negative staining. (Heat-induced epitope retrieval by Tris-EDTA buffer (pH8.0) at 120°C for 2.5 min) (1:150).

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  Immunohistochemical staining of paraffin-embedded mouse cerebrum tissue within the normal limits using anti-GPR17 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811665) (1:150)

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  Immunohistochemical staining of paraffin-embedded rat cerebrum tissue within the normal limits using anti-GPR17 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811665) (1:150)

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Specifications

Product Data

• Clone Name: OTI6B9
• Applications: IHC
• Recommended Dilution: IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human GPR17 N-terminal. The exact sequence is proprietary.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens G protein-coupled receptor 17 (GPR17), transcript variant 2, mRNA.
• Database Link:
  NP_005282
  Entrez Gene 574402 MouseEntrez Gene 767613 RatEntrez Gene 2840 Human
  UniProt ID Q13304
• Synonyms: DKFZp686M18273

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane